3IBK

Crystal structure of a telomeric RNA quadruplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	105
Detector technology	CCD
Collection date	2009-06-08
Detector	OXFORD TITAN CCD
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	57.583, 57.583, 38.377
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	11.690 - 2.200
R-factor	0.216
Rwork	0.215
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k8p
RMSD bond length	0.013
RMSD bond angle	0.859
Data reduction software	CrysalisPro
Data scaling software	SCALA (3.3.9)
Phasing software	REFMAC (5.5.0072)
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.869	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	3912
<I/σ(I)>		4.54
Completeness [%]	99.4	97.3
Redundancy	6.3	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	285	MPD, , KCl, NaCl, NaCacodylate, polyamine, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K