3IB7
Crystal structure of full length Rv0805
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-28 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.008 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 100.178, 100.178, 80.248 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.400 - 1.600 |
| R-factor | 0.161 |
| Rwork | 0.159 |
| R-free | 0.17700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hy1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.162 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.057 | 0.528 |
| Number of reflections | 54354 | |
| <I/σ(I)> | 11.6 | 2.3 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 9.4 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Bis-Tris, amm. acetate, MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
