3IB6
Crystal structure of an uncharacterized protein from Listeria monocytogenes serotype 4b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.122, 125.970, 73.385 |
| Unit cell angles | 90.00, 103.58, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.221 |
| Rwork | 0.218 |
| R-free | 0.28200 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.529 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.101 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.115 | 0.463 |
| Number of reflections | 36797 | |
| <I/σ(I)> | 6.3 | 2.1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 16% PEG 3350, 100mM ammonium chloride, pH 7.0, vapor diffusion, temperature 294K |
