3IAG
CSL (RBP-Jk) bound to HES-1 nonconsensus site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-01 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 63.451, 93.137, 112.569 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.800 - 2.000 |
| R-factor | 0.19931 |
| Rwork | 0.197 |
| R-free | 0.24336 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.460 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.3.0020) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.055 | 0.038 | 0.337 |
| Number of reflections | 44865 | ||
| <I/σ(I)> | 19.8 | ||
| Completeness [%] | 99.1 | 93.9 | 100 |
| Redundancy | 5.5 | 4.9 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | under oil microbatch | 277 | Magnesium Formate, PEG 3350, Xylitol, under oil microbatch, temperature 277K |
