3IA0
Ethanolamine Utilization Microcompartment Shell Subunit, EutS-G39V mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 1 |
| Unit cell lengths | 73.581, 151.394, 127.456 |
| Unit cell angles | 90.00, 90.39, 90.00 |
Refinement procedure
| Resolution | 58.881 - 2.500 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i96 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.926 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.2) |
| Refinement software | PHENIX (1.4_4) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.000 | 60.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.166 | 0.109 | 0.645 |
| Number of reflections | 173054 | ||
| <I/σ(I)> | 4.2 | ||
| Completeness [%] | 90.8 | 86.3 | 92.7 |
| Redundancy | 2.7 | 2.9 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 298 | 0.1M sodium/potassium phosphate, 12% PEG3000, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
