3I9X
Crystal structure of a mutT/nudix family protein from Listeria innocua
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 96.129, 96.129, 97.594 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.27600 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.653 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.668 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.105 | 0.449 |
| Number of reflections | 14075 | |
| <I/σ(I)> | 26.4 | 9.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 42.4 | 43.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 294 | 100mM Hepes pH 7.5, 4% glycerol, 1.3M tri-sodium citrate, vapor diffusion, temperature 294K |
