3I9T

Crystal structure of the rat heme oxygenase (HO-1) in complex with heme binding dithiothreitol (DTT)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	95
Detector technology	CCD
Collection date	2005-05-26
Detector	RIGAKU JUPITER 210
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	65.739, 65.739, 120.663
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.150
R-factor	0.16467
Rwork	0.162
R-free	0.20855
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ivj
RMSD bond length	0.025
RMSD bond angle	1.916
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.150
Number of reflections	17047

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	4M Sodium Formate, 5mM Sodium azide, 100mM DTT, pH7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K