3I9S
Structure from the mobile metagenome of V.cholerae. Integron cassette protein VCH_CASS6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-24 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 75.239, 75.239, 310.424 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.310 - 2.200 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.23300 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.991 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.4_77)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.440 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.157 | 0.925 |
| Number of reflections | 46726 | |
| <I/σ(I)> | 8.3 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.2 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 294 | 0.1M Bis-tris, 1.5M NH4SO4, In situ proteolysis using 1/1000chymotrypsin. Cryo: paratone-N oil, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
