3I9F
Crystal structure of a putative type 11 methyltransferase from Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 116.728, 116.728, 116.728 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.25700 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.534 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.913 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.154 | 0.803 |
| Number of reflections | 18652 | |
| <I/σ(I)> | 15.1 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 18.9 | 19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 20% PEG 3350, 200mM potassium nitrate, pH 7.0, vapor diffusion, temperature 294K |
