3I8Z
Crystal structure of human chromobox homolog 4 (CBX4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97800 |
| Spacegroup name | I 41 |
| Unit cell lengths | 58.606, 58.606, 32.813 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.510 |
| R-factor | 0.229 |
| Rwork | 0.228 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h91 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.227 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
| Rmerge | 0.082 | 0.085 | 0.224 |
| Number of reflections | 8902 | ||
| <I/σ(I)> | 17.9 | ||
| Completeness [%] | 98.4 | 97.1 | 86.8 |
| Redundancy | 6.9 | 6.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 20% Isopropanol, 20% PEG 4000, 0.1 M Na Citrate pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
