3I8N
A domain of a conserved functionally known protein from Vibrio parahaemolyticus RIMD 2210633.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 69.301, 69.301, 255.136 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.036 - 2.145 |
| R-factor | 0.2212 |
| Rwork | 0.219 |
| R-free | 0.26450 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.173 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.145 | 2.150 |
| Rmerge | 0.150 | 0.736 |
| Number of reflections | 20653 | |
| <I/σ(I)> | 45.3 | 4.16 |
| Completeness [%] | 98.8 | 100 |
| Redundancy | 15.6 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 0.1M Bis-Tris Propane 2.0M Sodium Format, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
