3I86
Crystal structure of the P60 Domain from M. avium subspecies paratuberculosis antigen MAP1204
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 97.107, 97.107, 97.107 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.428 - 2.400 |
| R-factor | 0.2232 |
| Rwork | 0.220 |
| R-free | 0.27960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gt2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.210 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.430 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.169 | 0.856 |
| Number of reflections | 12254 | |
| <I/σ(I)> | 14.09 | 2.2 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 13 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 22% PEG 3350, 18% 2-propanol, 0.1M NaHEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
