3I7W
High pressure structure of wild-type RNase A (0.67 GPa)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | ENRAF-NONIUS FR590 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2008-01-24 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 0.7107 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 63.552, 63.552, 63.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.350 |
| R-factor | 0.252 |
| Rwork | 0.252 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fs3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.180 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.480 |
| High resolution limit [Å] | 2.350 | 5.040 | 2.350 |
| Rmerge | 0.135 | 0.472 | 0.397 |
| Rmeas | 0.574 | ||
| Number of reflections | 6408 | ||
| <I/σ(I)> | 4 | ||
| Completeness [%] | 99.4 | 100 | 96.5 |
| Redundancy | 1.8 | 11 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | ammonium sulfate, sodium chloride, sodium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
