3I7R
Dihydrodipicolinate synthase - K161R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-10-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 121.435, 121.435, 111.037 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.920 - 2.100 |
| R-factor | 0.19653 |
| Rwork | 0.195 |
| R-free | 0.23013 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yxc |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.284 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.920 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.114 | 0.443 |
| Number of reflections | 53975 | |
| <I/σ(I)> | 8.8 | 3.3 |
| Completeness [%] | 97.2 | 100 |
| Redundancy | 7.07 | 7.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10 | 276 | 1.8M POTASSIUM PHOSPHATE, PH 10, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 276K |
