3I7Q
Dihydrodipicolinate synthase mutant - K161A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-10-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 121.044, 121.044, 110.501 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.840 - 2.000 |
| R-factor | 0.20555 |
| Rwork | 0.204 |
| R-free | 0.23674 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yxc |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.349 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.840 | 2.070 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.910 | 0.453 |
| Number of reflections | 63475 | |
| <I/σ(I)> | 7.1 | 2.3 |
| Completeness [%] | 99.4 | 96.7 |
| Redundancy | 4.23 | 4.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10 | 276 | 1.8M POTASSIUM PHOSPHATE, PH 10, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 276K |
