3I7M
N-terminal domain of Xaa-Pro dipeptidase from Lactobacillus brevis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-29 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 53.867, 50.962, 49.289 |
| Unit cell angles | 90.00, 104.33, 90.00 |
Refinement procedure
| Resolution | 36.500 - 1.460 |
| R-factor | 0.164 |
| Rwork | 0.163 |
| R-free | 0.19800 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.791 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.500 | 1.490 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.053 | 0.550 |
| Number of reflections | 22642 | |
| <I/σ(I)> | 14.6 | 2.05 |
| Completeness [%] | 99.9 | 98.8 |
| Redundancy | 9.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.1 M Tris buffer, 0.2 M ammonium sulfate, 25% PEG-3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
