3I7K

Crystal Structure of DDB1 in Complex with the H-Box Motif of WHX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	173
Detector technology	CCD
Collection date	2008-05-28
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0,1.005
Spacegroup name	P 21 21 21
Unit cell lengths	64.081, 132.215, 184.001
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.030 - 2.800
R-factor	0.232
Rwork	0.230
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b5m
RMSD bond length	0.013
RMSD bond angle	1.598
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.030	2.870
High resolution limit [Å]	2.800	2.800
Rmerge	0.070	0.426
Number of reflections	35137
<I/σ(I)>	23.4	2.6
Completeness [%]	90.7	94.6
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	16% PEG 4000, 0.2M SODIUM CHLORIDE, 0.1M MES, 0.005 M DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K