3I6V
Crystal structure of a periplasmic His/Glu/Gln/Arg/opine family-binding protein from Silicibacter pomeroyi in complex with lysine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 70.931, 70.931, 141.359 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.22400 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.425 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.699 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.111 | 0.464 |
| Number of reflections | 25211 | |
| <I/σ(I)> | 17.8 | 6.4 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 17.6 | 17.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 294 | 100mM sodium acetate pH 4.6, 25% PEG 6K, vapor diffusion, temperature 294K |
