3I6P
Ethanolamine Utilization Microcompartment Shell Subunit, EutM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 99.480, 99.480, 113.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.950 - 2.100 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bn4 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.346 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.097 | 0.063 | 0.493 |
| Number of reflections | 34162 | ||
| <I/σ(I)> | 11 | ||
| Completeness [%] | 99.7 | 98.2 | 100 |
| Redundancy | 7.6 | 7.1 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.05M MES, 0.015M magnesium sulfate, 8% PEG 4000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
