3I6J
Ribonuclease A by Classical hanging drop method after high X-Ray dose on ESRF ID14-2 beamline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.3 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.362, 64.362, 63.745 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.960 - 1.300 |
| R-factor | 0.21 |
| Rwork | 0.209 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2blp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.102 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.739 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.095 | 0.140 |
| Total number of observations | 28846 | |
| Number of reflections | 37998 | |
| <I/σ(I)> | 12.3 | 0.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 1.75M ammonium sulphate, 2.0M Sodium Chloride, 100mM Na-acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
