3I62

Structure of Mss116p bound to ssRNA and ADP-Aluminum Fluoride

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2008-12-15
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97857
Spacegroup name	P 21 21 2
Unit cell lengths	88.826, 126.111, 55.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.570 - 1.950
R-factor	0.17946
Rwork	0.178
R-free	0.20860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3i5x
RMSD bond length	0.007
RMSD bond angle	1.031
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC (5.5.0070)
Refinement software	REFMAC (5.5.0070)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.082	0.419
Number of reflections	44781
<I/σ(I)>	29.8	2.7
Completeness [%]	96.4	71.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	0.2 M succinate, 15% PEG 3350 , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K