3I61

Structure of Mss116p bound to ssRNA and ADP-Beryllium Fluoride

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2008-12-15
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97857
Spacegroup name	P 21 21 2
Unit cell lengths	88.294, 126.082, 55.721
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.370 - 2.100
R-factor	0.18803
Rwork	0.186
R-free	0.22136
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3i5x
RMSD bond length	0.007
RMSD bond angle	1.056
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC (5.5.0070)
Refinement software	REFMAC (5.5.0070)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.093	0.398
Number of reflections	36177
<I/σ(I)>	21.8	3.5
Completeness [%]	98.1	87.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	8% tacsimate, 15% PEG 3350, 2.5% ethylene glycol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K