Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Collection date | 2005-11-05 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 194.385, 100.261, 80.600 |
| Unit cell angles | 90.00, 105.31, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.400 |
| Rwork | 0.262 |
| R-free | 0.33580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2OVK |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.400 |
| Rmerge | 0.172 |
| Number of reflections | 19652 |
| <I/σ(I)> | 7.5 |
| Completeness [%] | 95.7 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 277 | 8% PEG 5000 MME, 150MM NACL, 100MM HEPES, 5% ETHYLENE GLYCOL, 5MM MGAC2, 2MM CAAC2, 2MM NAN3, 2MM BETA-MERCAPTOETHANOL, PH 7.6VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277K |
