Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Collection date | 2005-08-15 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 194.138, 101.751, 80.651 |
| Unit cell angles | 90.00, 105.74, 90.00 |
Refinement procedure
| Resolution | 46.730 - 2.600 |
| Rwork | 0.252 |
| R-free | 0.29710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s5g |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.538 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.730 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.097 |
| Number of reflections | 42948 |
| <I/σ(I)> | 11.2 |
| Completeness [%] | 94.7 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 277 | 8% PEG 5K MME, 150MM NACL, 100MM HEPES (PH 7.6), 5% ETHYLENE GLYCOL, 5MM MGAC2, 2MM CAAC2, 2MM NAN3, 2MM BETA-MERCAPTOETHANOL, 50MM NA MALONATE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277K |
