3I5B
Crystal structure of the isolated GGDEF domain of WpsR from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-10 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9771 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.615, 94.497, 97.592 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.357 - 2.043 |
| R-factor | 0.198 |
| Rwork | 0.193 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.020 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Number of reflections | 21511 | |
| <I/σ(I)> | 18.5 | 5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Na-K-tartrate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
