3I5A
Crystal structure of full-length WpsR from Pseudomonas syringae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-10 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 111.700, 181.200, 39.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.543 - 2.796 |
| R-factor | 0.239 |
| Rwork | 0.238 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.093 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.960 |
| High resolution limit [Å] | 2.796 | 2.800 |
| Number of reflections | 10062 | |
| <I/σ(I)> | 16.1 | 3.9 |
| Completeness [%] | 96.2 | 89.5 |
| Redundancy | 8.3 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | NaCl, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
