3I4R

Nup107(aa658-925)/Nup133(aa517-1156) complex, H.sapiens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2007-12-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	115.554, 133.047, 176.303
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.630 - 3.530
R-factor	0.32
Rwork	0.315
R-free	0.37000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.835
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.4_4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.630
High resolution limit [Å]	3.500	3.500
Number of reflections	29866
<I/σ(I)>	11.6	0.59
Completeness [%]	85.7	40
Redundancy	5.4	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	291	0.8 M sodium/potassium phosphate, 15 % glycerol, 2% PEG 3350, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K