3I4O

Crystal Structure of Translation Initiation Factor 1 from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-06
Detector	MAR CCD 165 mm
Wavelength(s)	0.9749
Spacegroup name	P 21 21 2
Unit cell lengths	73.950, 76.540, 28.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.300 - 1.470
R-factor	0.16
Rwork	0.159
R-free	0.18200
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.566
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.270	30.000	1.500
High resolution limit [Å]	1.470	3.990	1.470
Rmerge	0.086	0.052	0.359
Number of reflections	27447
<I/σ(I)>	16.5
Completeness [%]	98.2	83.8	97
Redundancy	7.2	5.8	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	292	12-16% (w/v) PEG 8000, 50-200mM KCl and 8-16% (v/v) glycerol, pH 4.5, vapor diffusion, hanging drop, temperature 292K
1	VAPOR DIFFUSION, HANGING DROP	4.5	292	12-16% (w/v) PEG 8000, 50-200mM KCl and 8-16% (v/v) glycerol, pH 4.5, vapor diffusion, hanging drop, temperature 292K