3I49
Crystal Structure of AlkB in complex with Fe(II), 2-oxoglutarate and methylated trinucleotide T-meC-T
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Detector technology | CCD |
| Collection date | 2006-11-15 |
| Wavelength(s) | 0.97947 |
| Spacegroup name | P 43 |
| Unit cell lengths | 41.374, 41.374, 117.213 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.800 - 1.600 |
| Rwork | 0.186 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | COMO (1.2) |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 4.340 | 1.600 |
| Rmerge | 0.074 | 0.027 | 0.656 |
| Number of reflections | 25984 | ||
| <I/σ(I)> | 8.2 | ||
| Completeness [%] | 99.9 | 99.3 | 100 |
| Redundancy | 7.9 | 8.4 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 3350, sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
