3I3V
Crystal Structure of probable secreted solute-binding lipoprotein from Streptomyces coelicolor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-08 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 91.314, 91.314, 370.019 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.700 - 2.300 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.25900 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.127 | 0.630 |
| Number of reflections | 79106 | |
| <I/σ(I)> | 8.3 | 2 |
| Completeness [%] | 98.9 | 91.3 |
| Redundancy | 9.1 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 2.1M DL-Malic Acid, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
