3I3U
Crystal structure of lp_1913 protein from lactobacillus plantarum, northeast structural genomics Consortium target lpr140a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.706, 60.287, 60.319 |
| Unit cell angles | 120.04, 89.96, 90.03 |
Refinement procedure
| Resolution | 26.120 - 2.800 |
| R-factor | 0.264 |
| Rwork | 0.264 |
| R-free | 0.29100 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.042 | 0.213 |
| Number of reflections | 36176 | |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 97.5 | 91 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | NaBr 0.1M, MES 0.1M, PEG 8000 20%, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
