3I3N
Crystal structure of the BTB-BACK domains of human KLHL11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.99590 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.093, 68.879, 136.834 |
| Unit cell angles | 90.00, 97.44, 90.00 |
Refinement procedure
| Resolution | 26.330 - 2.600 |
| Rwork | 0.260 |
| R-free | 0.28600 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.250 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.115 | 0.492 |
| Number of reflections | 23589 | |
| <I/σ(I)> | 13.3 | 3.1 |
| Completeness [%] | 99.6 | 98.2 |
| Redundancy | 6.2 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.25M K thiocyanate, 25w/v PEG 3350, 5v/v ethylene glycol, bis tris propane, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
