3I3F
Hypothetical protein from Giardia lamblia GL50803_14299
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-01 |
| Detector | ADSC Quantum-315R CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 119.900, 119.900, 104.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.350 - 1.350 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.893 |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.410 | 39.410 | 1.420 |
| High resolution limit [Å] | 1.350 | 4.270 | 1.350 |
| Rmerge | 0.074 | 0.027 | 0.522 |
| Total number of observations | 17266 | 55749 | |
| Number of reflections | 82643 | ||
| <I/σ(I)> | 13.5 | 22.1 | 1.4 |
| Completeness [%] | 99.5 | 98.8 | 96.6 |
| Redundancy | 6.3 | 6.2 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop, vapor diffusion | 5.5 | 290 | 100 mM Tris, pH 5.5, 25% PEG 3350, 200 mM ammonium acetate, sitting drop, vapor diffusion, temperature 290K |
