3I3F

Hypothetical protein from Giardia lamblia GL50803_14299

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-01
Detector	ADSC Quantum-315R CCD
Wavelength(s)	0.9795
Spacegroup name	I 41 2 2
Unit cell lengths	119.900, 119.900, 104.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.350 - 1.350
R-factor	0.177
Rwork	0.176
R-free	0.19100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.893
Data scaling software	SCALA (3.2.25)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.410	39.410	1.420
High resolution limit [Å]	1.350	4.270	1.350
Rmerge	0.074	0.027	0.522
Total number of observations		17266	55749
Number of reflections	82643
<I/σ(I)>	13.5	22.1	1.4
Completeness [%]	99.5	98.8	96.6
Redundancy	6.3	6.2	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	sitting drop, vapor diffusion	5.5	290	100 mM Tris, pH 5.5, 25% PEG 3350, 200 mM ammonium acetate, sitting drop, vapor diffusion, temperature 290K