3I38
Structure of a putative chaperone protein dnaj from klebsiella pneumoniae subsp. pneumoniae mgh 78578
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-18 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.805, 61.626, 92.907 |
| Unit cell angles | 81.75, 78.71, 83.79 |
Refinement procedure
| Resolution | 90.540 - 2.300 |
| R-factor | 0.219 |
| Rwork | 0.217 |
| R-free | 0.26200 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.743 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0051) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.540 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.559 |
| Number of reflections | 66358 | |
| <I/σ(I)> | 30.081 | 2 |
| Completeness [%] | 98.0 | 97.6 |
| Redundancy | 3.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | 0.2M Ammonium acetate, 0.1M Bis-Tris, 25% PEG 3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
