3I2Z
Structure of cold shock protein E from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.07400 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.395, 46.798, 46.718 |
| Unit cell angles | 90.00, 103.84, 90.00 |
Refinement procedure
| Resolution | 11.210 - 1.100 |
| R-factor | 0.20506 |
| Rwork | 0.204 |
| R-free | 0.23424 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 1MJC; A decapeptide (residues 12-21) from the E. coli cold shock protein A |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.935 |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.360 | 1.160 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.047 | 0.392 |
| Total number of observations | 260904 | |
| Number of reflections | 47339 | |
| <I/σ(I)> | 2.8 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 290 | 28% PEG 20000, 0.05M AMPSO, 1% glycerol, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
