3I2W
Crystal structure of EFC/F-BAR domain of Drosophila Syndapin/PACSIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.595, 85.525, 192.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.070 - 2.670 |
| R-factor | 0.2353 |
| Rwork | 0.234 |
| R-free | 0.25789 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.108 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.090 | 2.810 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rmerge | 0.111 | 0.377 |
| Number of reflections | 30043 | |
| <I/σ(I)> | 22.2 | 5.6 |
| Completeness [%] | 99.5 | 96.7 |
| Redundancy | 13.6 | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 17% PEG 3350, 0.1M disodium phosphate, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
