3I24
Crystal Structure of a HIT family hydrolase protein from Vibrio fischeri. Northeast Structural Genomics Consortium target id VfR176
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.433, 69.312, 49.077 |
| Unit cell angles | 90.00, 109.44, 90.00 |
Refinement procedure
| Resolution | 27.740 - 1.500 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.21500 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.037 | 0.118 |
| Number of reflections | 79694 | |
| <I/σ(I)> | 20.9 | 18 |
| Completeness [%] | 91.7 | 98.5 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.15 | 293 | NaCl 200 mM, MES PH6, PEG3350 20%, pH 6.15, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
