3I1K
Structure of porcine torovirus Hemagglutinin-Esterase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.8726 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 156.307, 104.528, 97.473 |
| Unit cell angles | 90.00, 95.79, 90.00 |
Refinement procedure
| Resolution | 48.490 - 2.100 |
| R-factor | 0.17252 |
| Rwork | 0.171 |
| R-free | 0.20463 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cl4 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.567 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.116 | 0.721 |
| Number of reflections | 90957 | |
| <I/σ(I)> | 8.4 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2M potassium acetate, 18% (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
