3I17
Crystal structure of the apo R132K:L121E mutant of cellular retinoic acid-binding protein II at 1.68 angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.99999 |
Spacegroup name | P 1 |
Unit cell lengths | 34.699, 37.271, 60.371 |
Unit cell angles | 105.80, 106.44, 89.97 |
Refinement procedure
Resolution | 35.740 - 1.680 |
R-factor | 0.18259 |
Rwork | 0.180 |
R-free | 0.23095 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g7b |
RMSD bond length | 0.010 |
RMSD bond angle | 1.333 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.560 | 1.724 |
High resolution limit [Å] | 1.680 | 1.680 |
Rmerge | 0.051 | 0.251 |
Number of reflections | 30624 | |
<I/σ(I)> | 20.8 | 4 |
Completeness [%] | 94.5 | 90.95 |
Redundancy | 3.8 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | 0.1 M Bis-tris-propane, pH 9.5, 30% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |