3I0N
Structure of the S. pombe Nbs1 FHA/BRCT-repeat domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9757 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.189, 64.898, 106.363 |
| Unit cell angles | 90.00, 105.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.300 |
| R-factor | 0.21731 |
| Rwork | 0.215 |
| R-free | 0.26250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i0m |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.128 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | 0.404 |
| Number of reflections | 30800 | |
| Completeness [%] | 94.5 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 20% PEG3000, 0.1M sodium citrate, Protein reductively methylated/carboxypeptidase-treated, pH5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
