3I0K
Crystal structure of GTB C80S/C196S/C209S + UDP + H antigen
Experimental procedure
| Experimental method | Molecular Replacement |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-27 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.104, 150.410, 79.253 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.870 - 2.200 |
| R-factor | 0.17729 |
| Rwork | 0.175 |
| R-free | 0.22808 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lz7 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.202 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 22845 |
| Completeness [%] | 95.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 279.15 | 35mM sodium acetate pH 4.6, 45 mM ADA, 30mM sodium chloride, 3-4 mM magnesium chloride, 3-3.5% PEG 4000, vapor diffusion, hanging drop, temperature 279.15K |
