3I07
Crystal structure of a putative organic hydroperoxide resistance protein from Vibrio cholerae O1 biovar eltor str. N16961
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.026, 76.154, 79.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.16626 |
| Rwork | 0.165 |
| R-free | 0.18454 |
| Structure solution method | MIR |
| Starting model (for MR) | 3eer |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.425 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 0.290 |
| Number of reflections | 35847 | |
| <I/σ(I)> | 27 | 1.9 |
| Completeness [%] | 94.5 | 52.4 |
| Redundancy | 4 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.05 Cesium chloride 0.1 M MES 30% Jeffamine M-600, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
