3HZZ
2.4 Angstrom Crystal Structure of Streptomyces collinus crotonyl CoA carboxylase/reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-07-13 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.668, 134.380, 195.134 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.970 - 2.400 |
| R-factor | 0.20733 |
| Rwork | 0.206 |
| R-free | 0.25712 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.079 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.970 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.119 | 0.344 |
| Number of reflections | 78199 | |
| <I/σ(I)> | 15.3 | 7.4 |
| Completeness [%] | 94.7 | 92.5 |
| Redundancy | 11.26 | 11.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | vapor diffusion with microseeding | 6.2 | 293 | 0.1M MES, 2.0M Ammonium Sulfate, 3% PEG 200, pH 6.2, vapor diffusion with microseeding, temperature 293K |
