3HZR

Tryptophanyl-tRNA synthetase homolog from Entamoeba histolytica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-07-15
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	93.200, 158.880, 100.280
Unit cell angles	90.00, 107.33, 90.00

Refinement procedure

Resolution	47.890 - 3.000
R-factor	0.229
Rwork	0.228
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3foc
RMSD bond length	0.012
RMSD bond angle	1.361
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Phasing software	BALBES
Refinement software	REFMAC (refmac_5.5.0089)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.891	48.620	3.160
High resolution limit [Å]	3.000	9.490	3.000
Rmerge	0.115	0.010	0.010
Total number of observations		5510	25116
Number of reflections	54415
<I/σ(I)>	5.609	13.7	0.7
Completeness [%]	97.7	93.5	98.8
Redundancy	3.2	3.3	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	0.2 M Sodium Citrate, 20% PEG 3350, 1mM TCEP, 10 mM Zn, pH 7.0, vapor diffusion, temperature 293K