3HZQ
Structure of a tetrameric MscL in an expanded intermediate state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-23 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 138.589, 138.589, 138.589 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.010 - 3.820 |
| R-factor | 0.292 |
| Rwork | 0.292 |
| R-free | 0.31200 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.890 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.040 | 0.776 |
| Number of reflections | 2395 | |
| <I/σ(I)> | 41.8 | 3.3 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 14 | 12.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 24-30% PEG400, 100 mM Tris-HCl (pH7.0), 150 mM Na2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
