3HZN
Structure of the Salmonella typhimurium nfnB dihydropteridine reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-03-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 280.340, 96.760, 131.840 |
| Unit cell angles | 90.00, 90.36, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.22200 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.092 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.124 | 0.510 |
| Number of reflections | 136466 | |
| <I/σ(I)> | 13.722 | 3 |
| Completeness [%] | 99.5 | 97.5 |
| Redundancy | 7.2 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 60% tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
