3HZJ

Crystal structure of the RabGAP domain of the RABGAP1L protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-18
Detector	MARCCD 300
Wavelength(s)	0.92015
Spacegroup name	P 21 21 21
Unit cell lengths	48.086, 64.571, 290.255
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.245
Rwork	0.244
R-free	0.27400
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.117
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.053	0.035	0.233
Number of reflections	38070
<I/σ(I)>	31.893
Completeness [%]	88.4	99.3	49.8
Redundancy	3.5	4.1	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6	291	16% PEG3350, 0.2M potassium chloride, 1:100 (w/w) Dispase., pH 6.0, vapor diffusion, hanging drop, temperature 291K
1		6	291	16% PEG3350, 0.2M potassium chloride, 1:100 (w/w) Dispase., pH 6.0, vapor diffusion, hanging drop, temperature 291K