3HZ8
Crystal structure of the oxidized T176V DsbA1 mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97973 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.307, 47.615, 79.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.450 |
| R-factor | 0.19452 |
| Rwork | 0.191 |
| R-free | 0.22625 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3dvw |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.171 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC (5.2.0019) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 1.520 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.061 | 0.264 |
| Number of reflections | 30676 | |
| <I/σ(I)> | 6.2 | 3 |
| Completeness [%] | 99.4 | 96.4 |
| Redundancy | 6.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9 | 293 | Citrate, pH 9.0, VAPOR DIFFUSION, temperature 293K |
