3HZ3
Lactobacillus reuteri N-terminally truncated glucansucrase GTF180(D1025N)-sucrose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.782, 66.233, 82.724 |
| Unit cell angles | 107.13, 101.37, 85.40 |
Refinement procedure
| Resolution | 20.000 - 2.220 |
| R-factor | 0.19531 |
| Rwork | 0.193 |
| R-free | 0.24340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.419 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.3.0011) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Number of reflections | 54782 | |
| <I/σ(I)> | 15.6 | 4.4 |
| Completeness [%] | 96.2 | 89.3 |
| Redundancy | 3.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 3350, BIS-TRIS propane buffer, pH 6.5, vapor diffusion, hanging drop, temperature 293K |
