3HZ2

Crystal structure of a betagamma-crystallin from an Archaea

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-01-29
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	29.300, 54.202, 54.179
Unit cell angles	85.81, 74.31, 74.32

Refinement procedure

Resolution	25.000 - 1.860
Rwork	0.172
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bv2
RMSD bond length	0.005
RMSD bond angle	1.090
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	1.930
High resolution limit [Å]	1.860	4.000	1.860
Rmerge	0.037	0.025	0.105
Number of reflections	24461
<I/σ(I)>	28.857		8.07
Completeness [%]	94.1	98.7	86.4
Redundancy	2.6	2.6	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	12% PEG 20000, 0.1M NaMES, pH6.5, temperature 298K, VAPOR DIFFUSION, SITTING DROP